FIGURE 4.

NF-κB reporter assays for the vFLIP mutant in which all 5 out of 6 cysteines were mutated to serine. A, Western blot analysis of IKKγ expression level in IKKγ knock-out mouse PreB cell line 1.3E2, respective parental cells 70Z/3 and IKKγ wild type or mutant reconstituted 1.3E2 cells. B, NF-κB activation was measured in the above-mentioned cell lines using the BrightGlo Luciferase assay system, 6 h after stimulation with LPS (10 μg ml⁻¹) or 48 h after transduction with KSHV vFLIP LV (MOI = 50). Bars represent mean fold induction values ± S.D.