Skip to main content
. 2015 May 22;290(27):16560–16572. doi: 10.1074/jbc.M115.653162

FIGURE 3.

FIGURE 3.

N-Glycans containing β1,2-xylose and α1,3-fucose residues are decreased in fab and xylt-1. A–D, MALDI-TOF mass spectra of the permethylated total N-glycans extracted from Col-0, fab, xylt-1, and fabx, respectively. The highest peak area from each spectrum was set to 100% for direct comparison of the amounts of N-glycans in each plant. Measured masses are given above or beside the peaks with illustrations of the N-glycan structures. E, relative amounts of N-glycans in Col-0, fab, xylt-1, and fabx. The relative amounts of the N-glycans were compared with the peak area ratios calculated based on that of the internal standard ([M + Na]+ = 2757.3761, theoretical m/z). Illustrations of the N-glycan structures are shown at the bottom of the graph. GlcNAc, mannose, xylose, and fucose residues are represented with dark blue, green, brown, and dark red, respectively. Relative amounts of N-glycans in Col-0, fab, xylt-1, and fabx are shown as red, yellow, green, and blue bars, respectively. Error bars indicate S.D.