FIGURE 11.

Hc-CATH binds to MPMs and blocks TLR4. A, MPM cells were incubated with FITC-Hc-CATH or FITC-sHc-CATH (2 μg/ml) for 15 min at 37 °C before assay. B, Hc-CATH binds to TLR4 on the surface of MPMs. MPM cells were preincubated with Hc-CATH or sHc-CATH (2 μg/ml) at 37 °C for 15 min and then were stained with anti-mouse PE-TLR4 (2 μg/ml) for 30 min at 4 °C.