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. 2015 May 26;290(27):16633–16652. doi: 10.1074/jbc.M115.642645

FIGURE 5.

FIGURE 5.

Stability of Hc-CATH. A, salt tolerance of Hc-CATH. MICs of Hc-CATH against E. coli ATCC25922 are shown in the presence of 0, 50, 100, 150, 200, and 400 mm sodium chloride. B, thermal tolerance of Hc-CATH. Hc-CATH solution (2 mg/ml, dissolved in sterile deionized water) was incubated at different temperatures (4, 20, 37, 50, 70, and 90 °C) for 1 h, and then the MICs against E. coli ATCC25922 were determined. C, thermal stability of Hc-CATH solution at 37 °C. Hc-CATH solution was incubated at 37 °C for 0 to 96 h, and then the MICs against E. coli ATCC25922 were determined. D, effect of human serum on the antimicrobial activity of Hc-CATH. Hc-CATH was incubated with human serum (1:4 (v/v)) at 37 °C for 0 to 6 h, and MICs of the samples against E. coli ATCC25922 were determined at each time point. The results represent the mean values of three independent experiments.