FIGURE 2.

Hypo-osmotic stress reduces FRET between Cav1 molecules. A, FRET between GFP-Cav1 and mCherry-Cav1 in A10 cells under iso-osmotic conditions (upper panels) and 5 min after the osmolarity is reduced to 150 mosm (lower panels). B, changes in FRET before (control) and 5 min after a reduction of osmolarity from 300 to 150 mosm (swollen). Values designated by R refer to FRET measurements taken after the cells were returned to 300 mosm for 3 min and 24, 48, and 72 h (R-3min, R-24 h, R-48 hrs, and R-72 hrs, respectively). The inset shows a comparison of the FRET values for cells at 150 mosm (●) and controls at 300 mosm (○) over the 72-h period. The data are an average of over 36 cells, and S.D. is shown.