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Osmotic stress reduces association between Gαq and Cav1. A, coimmunofluorescence studies showing the colocalization between Cav1 (green) and Gαq (red) at iso-osmotic (300 mosm) and hypo-osmotic (150 mosm) conditions in A10 cells (left). The compiled data were from three separate experiments where n = 17–33 for each experiment (right). B, FRET between eCFP-Gαq and eYFP-Cav1 with osmotic stress where n = 30.
