FIGURE 7.

Ca²⁺/CaM binding to ¹⁵N-labeled Par17. A, central region of superposed ¹H,¹⁵N TROSY spectra collected at 25 °C and pH 6.8 with ¹⁵N-labeled Par17 (0.6 mm) in the absence of Ca²⁺/CaM (black) and in presence of a 1.4-fold molar Ca²⁺/CaM excess (red). B, CSP effects in Par17 upon Ca²⁺/CaM binding are shown as blue and magenta bars, indicating either signal shifts or extreme line broadening, respectively. C, helical wheel representation of the predicted amphipathic α-helix at the N terminus of Par17. Hydrophobic, polar, acidic, and basic residues are highlighted in yellow, pink, red, and blue, respectively. The arrow indicates the hydrophobic moment of the helix (65); the N- and C-terminal ends of the wheel are marked by green capital letters.