FIGURE 9.

Par17 binding to ¹⁵N-labeled Ca²⁺/CaM. A, superposed ¹H,¹⁵N TROSY spectra collected at 25 °C and pH 6.8 with ¹⁵N-labeled Ca²⁺/CaM (0.5 mm) in the absence of Par17 (blue) and in presence of Par17 at a 5-fold (magenta) and 2-fold (red) molar excess of Ca²⁺/CaM. B, superposed ¹H,¹⁵N TROSY spectra collected at 25 °C and pH 6.8 with ¹⁵N-labeled Ca²⁺/CaM (0.5 mm) without Par17^2–22 (blue), with Par17^2–22 at 1:1 molar ratio (magenta), and with Par17^2–22 in 3-fold molar excess (red). The CSP effects observed in Ca²⁺/CaM upon binding of Par17 (at 2:1 molar stoichiometry) and Par17^2–22 (at 1:3 molar stoichiometry) are shown as insets in panels A and B, respectively.