FIGURE 4.

Competition results to define primary sugar-binding site in BDCA-2. Binding was performed with biotin-tagged CRD from BDCA-2 bound to streptavidin-coated wells. A and B, binding of ¹²⁵I-Man-BSA or ¹²⁵I-IgG was measured in the presence of competing α-methyl glycosides. C, binding of 125I-Man-BSA was measured in the presence of competing IgG. Half-maximal inhibition values (K_I) were obtained by nonlinear least squares fitting of the data. The values of K_I relative to the K_I for α-methyl mannoside in bar graphs represent the means ± standard deviations for two to four independent assays.