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. 2015 May 19;290(27):16841–16850. doi: 10.1074/jbc.M114.620799

FIGURE 2.

FIGURE 2.

Single-molecule motility assay with wt Cin8. A and B, kymographs of single-molecule assays of wt Cin8 on surface-immobilized MTs. A, in high-ionic-strength buffer. B, in low-ionic-strength buffer. Representative kymographs are shown. The Atto 488-labeled minus-ends of the MTs are on the left side in all panels. Vertical scale bars: 8 s; horizontal scale bars: 3 μm. C and D, histograms of velocities obtained from segmentation analysis of kymographs for wt Cin8. C, average velocity for high-ionic-strength buffer. vavg = −256 ± 7 nm/s (mean ± S.E.); number of segments, n = 502. D, average velocities for low-ionic-strength buffer. Overall average, vavg = 10 ± 4 nm/s; plus-end average (red), v+ = 80 ± 3 nm/s, n = 166; and minus-end average (gray), v− = −73 ± 3 nm/s, n = 133. Velocities close to zero, in the range between −25 nm/s and 25 nm/s, are indicated in green. E, control, histogram of apparent velocities of immobile Cin8 motors (straight lines in kymographs). The width of the Gaussian fit to this distribution is 34 nm/s.