Lack of AQP4 translocation response in the U373 glioblastoma cell-line.
A and B, representative confocal micrographs of an AQP4-GFP-transfected U373 cell exposed to isotonic (340 mosm/kg of H2O) media (A) and 5-min exposure to hypotonic (85 mosm/kg of H2O) media (B). C and D, cell surface biotinylation of U373 cells transfected with AQP4 (C) and AQP4-GFP (D). No statistically significant change was observed between any of the treatment groups (340, 170, and 85 mosm/kg of H2O), n = 3. Data are presented as mean ± S.E.