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. 2015 May 20;290(27):16906–16917. doi: 10.1074/jbc.M115.642363

FIGURE 3.

FIGURE 3.

TEAD binding domain is essential for TAZ-induced transcriptional repression of ΔNp63. A, TEAD binding domain is critical for ΔNp63 repression. Constitutively active TAZ-S89A with mutations in TEAD binding (S89A-F52A/F53A) or WW (S89A-WWm) domains were induced with Dox in MCF10A cells. ΔNp63 and TAZ protein expression was assessed and compared with MCF10A cells with inducible expression of TAZ-S89A (S89A). β-Actin was used as an internal loading control. B, qRT-PCR analysis of ΔNp63 mRNA in MCF10A cells expressing WPI, TAZ, or TAZ-F52A/F53A. C, TEAD binding mutant of TAZ abolishes TAZ-mediated suppression of ΔNp63 promoter. SK-BR3 cells were transfected with ΔNp63-luc alone or in combination with wild-type TAZ (TAZ) or its TEAD binding mutant (TAZ-F52A/F53A). Promoter activity was measured as described in Fig. 2E. The experiment was performed in triplicate. D, qRT-PCR analysis of TAZ-down-regulated genes in MCF10A-WPI, TAZ, or TAZ-F52A/F53A cells. Procedures and data analyses were performed as described in Fig. 1B. The experiment was performed in triplicate, and error bars represent S.D. from each set of triplicates. *, statistically significant difference (p < 0.05).