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. 2015 May 15;290(27):16989–17003. doi: 10.1074/jbc.M115.652818

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — CcmI and CcmE are required for maturation of class II cytochrome c′. A, SDS-PAGE/TMBZ heme staining using 100 μg of total soluble protein extracts from the appropriate R. capsulatus strains: 2 μg of purified cytochrome c′ as a positive control for heme staining (lane 1), wild type MT1131 (lane 2), CcmI-null strain MTSRP1 (lane 3), CcmE-null strain MD2 (lane 4), and cytochrome c₂-null strain MT-G4/S4 (lane 5). B, SDS-PAGE/immunoblot using anti-cytochrome c′ polyclonal antibodies with 100 μg of total soluble protein extracts from the appropriate R. capsulatus strains: 2 μg of purified cytochrome c′ as a positive control (lane 1), wild type MT1131 (lane 2), cytochrome c₂-null strain MTG4/S4 (lane 3), CcmI-null strain MTSRP1 (lane 4), and CcmE-null strain MD2 (lane 5).