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. 2015 May 5;290(25):15475–15486. doi: 10.1074/jbc.M115.636670

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Visualization of interactions between endogenous Mgats and UDP-sugar transporters using in situ PLA. A and B, PLA analysis of either Mgat1 (panels 1), Mgat2 (panels 2), Mgat4B (panels 3), or Mgat5 (panels 4) interaction with UGT (A) and NGT (B) in PC-3 cell line. C, negative control for Mgats-UDP-sugar transporters interaction analysis, where primary antibody against Mgat1/Mgat2/Mgat4B/Mgat5 was not added during the PLA procedure. Negative control for Mgat-UDP-sugar transporters interaction analysis, where primary antibody against UGT/NGT was not added during the PLA procedure (panels 5). D, negative control for Mgat-UDP-sugar transporters interaction where both primary antibodies were omitted during the PLA procedure. Bars, 20 μm.