TABLE 3.

In vivo FLIM-FRET analysis of interactions between UDP-sugar transporters and Mgats

FRET combinations	n	τaverage	τshort	fshort	τlong	χ2
		ns	ns		ns
eGFP-Mgat1 alone	11	2.65 ± 0.03		0.0	2.65 ± 0.03	0.99 ± 0.05
eGFP-Mgat1 + mRFP-NGT	25	2.40 ± 0.05	1.26 ± 0.10	0.18 ± 0.04	2.65	1.00 ± 0.05
eGFP-Mgat1 + mRFP-UGT1	27	2.05 ± 0.30	1.29 ± 0.22	0.44 ± 0.20	2.65	1.04 ± 0.06
eGFP-Mgat1 + mRFP-UGT2	19	2.69 ± 0.07		0.0	2.69 ± 0.07	1.03 ± 0.03
eGFP-Mgat2 alone	13	2.65 ± 0.07		0.0	2.65 ± 0.07	1.00 ± 0.02
eGFP-Mgat2 + mRFP-NGT	18	2.30 ± 0.07	1.34 ± 0.07	0.27 ± 0.05	2.65	1.04 ± 0.06
eGFP-Mgat2 + mRFP-UGT1	33	2.21 ± 0.13	1.65 ± 0.27	0.51 ± 0.25	2.65	1.02 ± 0.07
eGFP-Mgat2 + mRFP-UGT2	22	2.65 ± 0.06		0.0	2.65 ± 0.06	1.05 ± 0.05
eGFP-Mgat4B alone	11	2.73 ± 0.02		0.0	2.73 ± 0.02	1.02 ± 0.07
eGFP-Mgat4B + mRFP-NGT	23	2.37 ± 0.07	1.58 ± 0.06	0.31 ± 0.05	2.73	0.97 ± 0.05
eGFP-Mgat4B + mRFP-UGT1	29	2.09 ± 0.20	1.64 ± 0.11	0.58 ± 0.14	2.73	1.02 ± 0.07
eGFP-Mgat4B + mRFP-UGT2	29	2.52 ± 0.02	1.53 ± 0.12	0.18 ± 0.03	2.73	1.01 ± 0.06
eGFP-Mgat5 alone	13	2.63 ± 0.02		0.0	2.63 ± 0.02	1.04 ± 0.01
eGFP-Mgat5 + mRFP-NGT	21	2.11 ± 0.07	1.38 ± 0.04	0.39 ± 0.07	2.63	1.00 ± 0.06
eGFP-Mgat5 + mRFP-UGT1	30	2.20 ± 0.10	1.75 ± 0.22	0.52 ± 0.20	2.63	1.03 ± 0.07
eGFP-Mgat5 + mRFP-UGT2	20	2.65 ± 0.03		0.0	2.65 ± 0.03	1.04 ± 0.06