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ATP inhibition of sGC in the basal state for WT and DM. Changes in activity with increasing concentrations of ATP for WT (A) and DM (B) sGC. Data are replotted as double reciprocal plots for WT (C) and DM (D). sGC (0.1 μg) wild type or variant was assayed for cyclase activity in 50 mm HEPES, pH 7.4, 6 mm MgCl2, and 1 mm DTT. Each curve was fit to mixed type inhibition with two independent binding sites (Equations 1–3). Error bars, S.E.
