TABLE 2.

Kinetic parameters of ATP inhibition of sGC determined by fitting Equations 5–7 to ATP inhibition data

The parameter α is the cooperativity coefficient. It describes the changes in affinity at the active site due to binding of ATP at the allosteric site. The parameters β and γ denote the relative activity of sGC bound to ATP and GTP at the allosteric site, respectively (see Scheme 2).

Parameter	WT	DM
Ksc (μm)	34.0 ± 7	34.0 ± 7
Ksa (mm)	4.6 ± 1.3	4.6 ± 1.3
Kic (mm)	0.20 ± 0.08	0.20 ± 0.08
Kia (mm)	1.0 ± 0.3	1.0 ± 0.3
α	0.21 ± 0.04	0.35 ± 0.09
β	0.15 ± 0.03	0.45 ± 0.04
γ	0	0