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. 2015 May 5;290(25):15581–15594. doi: 10.1074/jbc.M115.650994

FIGURE 4.

FIGURE 4.

Effect of FoxO6 depletion on hepatic gluconeogenesis and insulin sensitivity in high fat-fed FoxO6-KO mice. A, pyruvate tolerance test. B, AUC. AUC was calculated from blood glucose profiles during pyruvate tolerance test. C, insulin tolerance test. Mice were injected intraperitoneally with regular human insulin (1.5 IU/kg) followed by determination of blood glucose levels. D, AUC. AUC was calculated from blood glucose profiles during insulin tolerance test. E, HOMA-IR. The HOMA-IR was determined by multiplying fasting blood glucose (mmol/liter) and fasting plasma insulin (μIU/ml) levels, divided by 22.5. F, fasting plasma insulin levels. Plasma insulin levels were determined after 16-h fasting. G, G6Pase mRNA levels. H, PEPCK mRNA levels. Data were obtained from high fat-fed male FoxO6-KO (n = 9) and age/sex-matched WT littermates (n = 8) at 20–22 weeks of age. Hepatic G6Pase and PEPCK mRNA levels were determined by real-time qRT-PCR using 18S RNA as control. *, p < 0.05 versus WT control.