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. 2015 May 5;290(25):15581–15594. doi: 10.1074/jbc.M115.650994

FIGURE 6.

FIGURE 6.

Effect of FoxO6 depletion on Kupffer cell content in the liver. Liver tissues were obtained from high fat-fed WT control (A) and FoxO6-KO mice (B) as described in Fig. 5. Frozen sections of liver tissues were subjected to anti-F4/80 immunohistochemistry. F4/80 positive cells (stained red) were counted and normalized to the total number of cells (with nuclei stained blue by DAPI) in liver sections (C). Aliquots of liver tissues (20 mg) were used for the preparation of total RNA, which was subjected to real-time qRT-PCR using 18S RNA as the control for determining F4/80, CD68, IL-1β, TNFα, IL6, CCR2, CCL2, CCL3, and CCL7 mRNA levels (D). Frozen sections (6 μm) of liver tissues from high fat-fed WT (E) and FoxO6-KO (F) mice were subjected to oil red O staining. In addition, aliquots of liver tissues (20 mg) were used for determining hepatic lipid content, which was defined as mg of triglyceride (TG) per gram of liver protein (G). *, p < 0.05 versus WT control. n = 8–9 per group. Bar, 50 μm.