TABLE 1.

Plasma lipid and cytokine profiles and hepatic G6P levels in high fat-fed FoxO6-KO and WT mice

High fat-fed FoxO6-KO (n = 9) and WT mice (n = 8) at 22 weeks of age were fasted for 16 h. Blood was sampled for the determination of plasma lipid and cytokine levels. Intracellular G6P levels in the liver, expressed as ng of G6P per mg of hepatic proteins, were determined by colorimetric G6P assay, as described under “Experimental Procedures.”

Blood parameters	WT	FoxO6-KO
Triglyceride (mg/dl)	64.8 ± 8.4	56.7 ± 2.8
Cholesterol (mg/dl)	201.2 ± 15.6	156.4 ± 10.7a
Free fatty acid (meq/liter)	0.66 ± 0.02	0.72 ± 0.04
CCL2 (pg/ml)	199 ± 42	184 ± 38
IL-6 (pg/ml)	9.3 ± 1.6	12.2 ± 2.7
TNFα (pg/ml)	4.8 ± 0.3	6.3 ± 1.2
G6P (ng/mg)	7,225 ± 451	6,055 ± 355a

^a p < 0.05 versus WT control.