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Stress agents stimulate human monocyte-derived DC to up-regulate ROS, HSP70. The optimum concentration of the stress agents used were sodium arsenite (As) 50 μm, gramicidin (Gr) 2 μm, dithiocarbamate (DITH) 1 μm, or alum 1%, which up-regulated (A) ROS, flow cytometry (B), intracellular HSP70 (C), flow cytometry (D), and cell surface HSP70 (E and F). G, HSP70 mRNA. All experiments were performed with 6 different samples of blood. *, p < 0.05; **, p < 0.01; ***, p < 0.001.
