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. 2015 Apr 24;290(25):15662–15669. doi: 10.1074/jbc.M115.646257

FIGURE 2.

FIGURE 2.

Gemin5 directly binds to the SMN mRNA. A, direct RNA binding assay performed with radiolabeled wild type SMN 3′-UTR or a control RNA sequence (as described in Fig. 1D). The RNA was bound to immunopurified Gemin5, Gemin3 or SMN protein or to a nonspecific isotype matched control IgG antibody. B, direct RNA binding assay as in A performed with 10 or 20 nucleotide truncations of the SMN 3′-UTR. For both, input represents 1% or 1,000 CPM of RNA used in each binding assay.