FIGURE 3.

Gemin5 binds to specific sequence and structure elements of the SMN 3′-UTR. A, mfold structure prediction for the 3′ fragment of the SMN 3′-UTR. B, RNA binding assay performed with radiolabeled SMN RNA that contain point mutations in the AU sequence as indicated above each lane. C, RNA binding assay performed with stem-loop deleted SMN RNA as indicated in A. Input and control are the same as described in Fig. 2. H1, helical region 1; H2, helical region 2; wt, wild type.