FIGURE 6.

Knockdown of SMN causes Gemin5-dependent up-regulation of SMN reporter. A, Western blot of streptavidin pulldown assay using cell extracts from control (nontargeting siRNA) or SMN knockdown cells. Control RNA used is nonspecific RNA transcribed from an empty pSP72 vector. Input is 10 μg of total protein from respective cell lines. B, dual luciferase reporter assay performed with a wild type SMN RNA (SMN 3′-UTR) or a Gemin5 binding sequence-deleted mutant of SMN (SMN mut 3′-UTR) expressed in cells also transfected with control or SMN RNAi. Ctrl, control; mut, mutant, *, p < 0.05.