FIGURE 4.

The effect of HSV-1 Us3 on T cell activation. Jurkat T cells were mock-infected or infected with wild-type or Us3-null HSV-1, followed by stimulation with OKT3. A, IL-2 mRNA was measured by real-time PCR and normalized to that of 18S rRNA. The level of IL-2 mRNA of each sample was calculated and is presented relative to the mock sample. B, the medium was collected, and secreted IL-2 was monitored by ELISA as described above. Non-stimulated sample (N.S., open columns) and OKT3-stimulated (closed columns) IL-2 measurements are presented as mean ± S.D. of three independent experiments. C, human CD3⁺ T cells were infected with the indicated viruses, and secretion of IL-2 was determined as described in B.