FIGURE 5.

Inhibition of TCR-mediated signaling by HSV-1. Jurkat T cells were mock-infected or infected with HSV-1 and the recombinant Us3-null virus. A, the phosphorylation of the indicated proteins was resolved by Western blot analysis with the corresponding total proteins. B, each band was quantified by densitometer, and the -fold of activation of each protein was calculated by the ratio of each phosphorylated band density to that of the non-stimulated band. Data are mean ± S.D. (n = 3). *, p < 0.05 by Student's t test. C, the infected cells were loaded with fluorescent dyes, and intracellular calcium was monitored as described under “Experimental Procedures.” The vertical axis displays the relative free calcium concentration, and the plot represents three independent experiments.