FIGURE 2.

The 18-amino acids of BCL-3-derivied peptides of interest were sequentially substituted with alanine, probed with GST-p50, and detected by immunoblotting with anti-GST antibody. The peptides are numbered in bold as described in the legend to Fig. 1. p50 binding was quantified by densitometry and calculated as a percentage binding of the parent unsubstituted peptide on the same array. Alanine substitutions that resulted in less than 60% binding are underlined. Corresponding BCL-3 amino acids are labeled above each individual substitution peptide series.