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. 2015 May 11;290(25):15717–15729. doi: 10.1074/jbc.M114.618355

FIGURE 9.

FIGURE 9.

Regulation of CsHMA5.1 and CsHMA5.2 in cucumber roots under different copper availability. A, the level of CsHMA5.1 and CsHMA5.2 transcripts in roots of 2-week-old cucumbers grown under copper excess or upon copper deficiency. Data represent means ± S.D. The letters indicate significant difference between CsHMA5.1 or CsHMA5.2 expression under different copper treatments (a) or between CsHMA5.1 and CsHMA5.2 expression under the same treatment (b). B, Western blot analysis of CsHMA5.1 and CsHMA5.2 proteins level in tonoplast isolated from roots of cucumbers grown under different copper treatments. Immunoblot of the same tonoplast fractions was probed with anti-PPase antibodies (vacuolar pyrophosphatase, V-PPase) to ensure equal loading of membrane protein onto SDS-PAGE. C, ATP-dependent copper transport into tonoplast vesicles isolated from roots of the same plants. Data represent means ± S.D. The letters (a) indicate significant difference between the active vacuolar copper accumulation under different copper treatments.