Skip to main content
. 2015 May 8;290(25):15758–15769. doi: 10.1074/jbc.M114.624353

FIGURE 7.

FIGURE 7.

Stress resistance is mediated by the UTRs but is not dependent on the IRES element. A, inducible expression of single CTSL cDNA variants in murine PyMT cells. Overview of expressed variants. The variant CTSL-A contains the complete described IRES motif. CTSL-A3 corresponds to the shortest described splice variant. CTSL-CDS does not contain any UTR. B, treatment scheme to assess newly synthesized CTSL under stress conditions. C, production of CTSL protein under hypoxia upon expression of different cDNA variants. D, production of CTSL protein under Torin-1 upon expression of different cDNA variants. E, treatment scheme to assess newly synthesized CTSL under Torin-1 and nocodazole treatment. F, production of cathepsin L protein under Torin-1 and nocodazole upon expression of different cDNA variants. G, polyribosome association of CTSL-CDS mRNA under Torin-1 treatment with/without addition of nocodazole.