FIGURE 3.

Fructose 1-phosphate (F1P) and sedoheptulose 1-phosphate (S1P) are synthesized from DHAP and glyceraldehyde and from erythrose, respectively, via aldolase. The synthesis of F1P from DHAP and glyceraldehyde (A) was carried out in triplicates as described (see “Experimental Procedures”). Reaction mixtures containing aldolase (0.00082 mg), DHAP, and glyceraldehyde were incubated at room temperature for 30 min, and the product formed was analyzed by LC-MS as described (see “Experimental Procedures”). The synthesis of S1P from DHAP and erythrose (B) was carried out in triplicates as described (see “Experimental Procedures”). Reaction mixtures containing aldolase (0.01 mg), DHAP, and erythrose were incubated at room temperature for 60 min, and the product formed was analyzed as described above. The products formed were expressed as nmol/min/mg aldolase. Error bars, S.D.