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. 2015 May 6;290(25):15825–15834. doi: 10.1074/jbc.M115.648428

FIGURE 2.

FIGURE 2.

A, time course of c-Src binding to the integrin β3 subunit and its subsequent activation after platelet stimulation by thrombin. Following the stimulation of washed human platelets by 1 unit/ml thrombin for the indicated times, platelets were lysed with 1% Nonidet P-40, β3 was immunoprecipitated (IP) from the lysates using a β3-specific monoclonal antibody, and the immunoprecipitated β3 was serially immunoblotted (IB) for β3, c-Src, and phosphorylated c-Src residue 419 (pTyr419). B, densitometry using NIH ImageJ software of the immunoblot shown in Fig. 2A demonstrating the time course of c-Src binding to the integrin β3 subunit and its subsequent activation after platelet stimulation by thrombin. The densitometry for β3-bound c-Src and for β3-bound c-Src containing phosphorylated tyrosine residue 419 (pY419) at each time point was normalized using the densitometry for the corresponding β3 band.