FIGURE 5.
Effect of treatment with PPi on the effects of MTS reagents on macroscopic current amplitude in different cysteine mutants. The mean effect of MTSES (A) and MTSET (B) (200 μm in each case) on macroscopic current amplitude at −50 mV is shown. In each case, gray bars represent currents recorded in the present of ATP and PKA alone, and black bars represent currents recorded following treatment with PPi (2 mm) in the presence of ATP and PKA. Asterisks indicate a significant difference between control and PPi-treated conditions (p < 0.05). Error bars represent the means ± S.E. from three to five patches.