FIGURE 3.

Co-expression of DAP12 with TREM2 increases the level of TREM2-ICD. A, HEK293T cells were transfected with TREM2-GFP then incubated for 16 h with 500 nm Compound E, 10 μm DAPT or vehicle control. Bar graphs to the right show the quantification of Western blots as ratios of TREM2-CTF/TREM2-FL or TREM2-ICD/TREM2-FL (n = 3). **, p < 0.01. B, BV2 cells were transfected with TREM2-GFP in combination with DAP12-Myc or vector control. Cells were harvested 24 h post-transfection, followed by Western blotting with antibodies against GFP (TREM2-FL, TREM2-CTF and TREM2-ICD), Myc (DAP12) and α-Tubulin. Bar graphs to the right show the quantification of Western blots as ratios of TREM2-CTF/TREM2-FL or TREM2-ICD/TREM2-FL (n = 3). **, p < 0.01; ***, p < 0.001.