FIGURE 7.

TREM2-CTF reduces LPS-induced pro-inflammatory cytokine production. A, BV2 cells were transiently transfected with TREM2-FL-Myc or TREM2-CTF-Myc and harvested for protein analysis 24 h post transfection. B, BV2 cells were transiently transfected with TREM2-FL-Myc and TREM2-CTF-Myc for 24 h. Following immunostaining with antibodies to TGN46, Myc or Giantin, cells were observed by fluorescence confocal microscopy with representative staining shown. Scale bar: 10 μm. C, cells from panel A were treated with 500 ng/ml LPS or vehicle control for 6 h. RNA was extracted and the relative mRNA levels of IL-1β and IL-6 shown as a bar graph were determined by quantitative RT-PCR (n = 4). β-Actin was used as an internal control. *, p < 0.05; **, p < 0.01; ***, p < 0.001.