TABLE 3.

Effect of different liprin-α3 fragments on the RhoA-mDia1 interaction dynamics as determined by stopped-flow analysis

The k_a is the association rate constant, and k_d is the dissociation rate constant.

Liprin fragment	ka	kd	KD
	μm−1 s−1	ms−1	nm
Without fragment	1.00 ± 0.01	2.35 ± 0.09	2.4
217–587	0.51 ± 0.01	2.82 ± 0.07	5.5
567–587	0.37 ± 0.01	3.22 ± 0.04	8.7
561–582	0.46 ± 0.01	3.22 ± 0.07	7.0
561–587	0.27 ± 0.01	2.84 ± 0.11	10.5