FIGURE 1.

Etoposide and teniposide induce CETP expression and secretion from HepG2 cells. A–D, HepG2 cells received the following treatment, and expression of CETP protein or mRNA was determined by Western blot or real time RT-PCR. A, etoposide or teniposide at the indicated concentrations for 16 h. B, etoposide (5 μm) or teniposide (500 nm) for the indicated times. C, etoposide or teniposide at the indicated concentrations for 12 h. D, etoposide (10 μm) or teniposide (100 nm) for the indicated times. *, versus control, p < 0.05 (n = 3). E, after the indicated treatment for 16 h, CETP protein expression was determined by immunofluorescent staining. F, HepG2 cells were treated with teniposide (0.5 μm) for the indicated times. The treatment medium was collected, concentrated, and normalized by cellular protein content before the secreted CETP protein was determined by Western blot.