Skip to main content
. 2015 Apr 25;290(23):14418–14429. doi: 10.1074/jbc.M115.643015

FIGURE 5.

FIGURE 5.

Etoposide and teniposide inactivate RIP140 pathway. A and B, HepG2 cells were treated with etoposide (Eto) or teniposide (Teni) at the indicated concentration for 12 and 16 h followed by determination of RIP140 mRNA and protein expression by real time RT-PCR and Western blot, respectively. *, versus control, p < 0.05 (n = 3). C, HepG2 cells were treated with teniposide and etoposide at the indicated concentrations for 12 h. Total cellular RNA was extracted and used to determine p21, Rb, and E2F1 mRNA expression by real time RT-PCR. *, versus control at p < 0.05 (n = 3). D, HepG2 cells were treated with teniposide at the indicated concentrations for 16 h. Expression of p21, E2F1, and pRb protein was determined by Western blot. E and F, 293T cells in 24-well plates were transfected with PGL-TK-Luc or PGL-3xE2F1-TK-Luc followed by treatment with teniposide and etoposide as indicated and determination of promoter activity. *, versus control in the corresponding group, p < 0.05 (n = 3). NS, not significant.