FIGURE 5.

Selective inhibition of ATB-BMPA photolabeling by compounds 3 and 17. DMSO (vehicle control) or compounds (Cmpd) 3 and 17 (20 μm) were added to 200 μg of LDM prepared from 3T3-L1 adipocytes for 10 min at room temperature. Samples were then irradiated with biotinylated ATB-BMPA (50 μm final concentration) as described under “Experimental Procedures.” Biotinylated proteins, isolated from detergent-solubilized LDM using a high capacity streptavidin-agarose resin, were analyzed by immunoblot analysis using GLUT4 or GLUT1 antibodies. GLUT proteins were quantified using an Odyssey Infrared Imaging System. Data, normalized to vehicle controls, represent the mean ± S.E. of three independent measurements. + and *, p < 0.05 versus GLUT1 or GLUT4 control, respectively.