FIGURE 8.

Structural derivatives of compounds 3 and 17 exhibit altered efficacy and selectivity. A, impact of analogues compounds (Cmpd) 26 and 39 (2 μm) on viability of MM cell lines after 72 h of treatment assessed by annexinV/DAPI staining. B, inhibition of 2-deoxyglucose uptake of GLUT4 and GLUT1 by compounds 26 and 39 in 293 cells. Compounds 26 and 39 (20 μm) were added 6 min prior to glucose uptake in 293 cells expressing GLUT1, GLUT4, and a GLUT4 transporter dead mutant P401L. 2-[³H]Deoxyglucose uptake was measured at 37 °C for 6 min as described under “Experimental Procedures.” Uptake from the GLUT4 P401L cells was subtracted from the corresponding GLUT1 and GLUT4 data and then normalized to vehicle control (Ctrl). Data shown as mean uptakes ± S.E. relative to control, n = 3; #, +, and *, p < 0.05.