FIGURE 1.
Reduced vasopressin action and increased copeptin in mTUGUBX-Cter mice. A and B, water intake was measured in 12-week-old control (WT) and mTUGUBX-Cter TG (TG) mice and is plotted per body weight (A) and per mouse (B). C, WT and TG mice were treated without food for 18 h, allowed to recover, and then treated without food and water for 18 h. The weight decrease attributable to water removal is plotted as a percentage of initial body weight. D, mice were sacrificed after removal of food and water, and kidney lysates were immunoblotted to detect the vasopressin-responsive protein AQP2 and, as a control, α-tubulin. The relative abundance of AQP2 is plotted in WT and TG mice. E, plasma concentrations of copeptin, a product of the vasopressin prohormone, were measured in fasted WT and TG mice with free access to water. Data are presented as mean ± S.E.; n = 6–12 in each group.