FIGURE 6.

The SNX-PXB proteins are localized to early endosomes via PtdIns3P interaction. A, HeLa cells were grown on coverslips and transfected with myc-SNX20, myc-SNX20-R113Q, or myc-SNX21, myc-SNX21-R161Q. Cells were co-labeled with anti-myc antibody with anti-rabbit Alexa 488 (green) and anti-EEA1 antibody or anti-Lamp1 antibody with anti-mouse Alexa 647 (red). Scale bars = 5 μm. B, native and mutant SNX20 were tested for binding against PtdIns3P by isothermal titration calorimetry and affinity measures are presented with standard deviation over three experiments. Raw data are at the top; integrated normalized data at the bottom.