TABLE 2.
SNX21 PXB/TPR | SNX20Δ50 | SNX21Δ93 | |
---|---|---|---|
Structural parametersa | |||
I(0) (cm−1) (Guinier/P(r)) | 0.031 | 0.050 | 0.041 |
Rg (Å) (Guinier) | 21.78 ± 0.28 | 24.37 ± 0.24 | 25.78 ± 0.08 |
Rg (Å) (P(r)) | 21.57 ± 0.25 | 24.41 ± 0.16 | 25.34 ± 0.07 |
Dmax (Å) | 67.0 ± 2.0 | 74 ± 2.0 | 84 ± 2.0 |
Molecular mass (Mr) determination | |||
Sequence (kDa) | 29.6 (dimer) | 31.0 | 40.8a |
MALLS | 29.3 | 28.9 | 40.6 |
SAXS | 30.6 | 33.2 | 42.1 |
Ab initio analysis | |||
χ (GASBOR) | 0.36 | 0.38 | 0.70 |
Rigid-body modeling/validation | |||
χ2 | 0.435 | 0.794 | 1.21 |
CRYSOL | BUNCH | SASREF |
a Including a 10-kDa ubiquitin tag.