TABLE 2.
Data of surface plasmon resonance kinetics fitted to a 1:1 binding model
RU, response units.
| Fragment | ka | kd | KD | Rmax | χ2 |
|---|---|---|---|---|---|
| 1/ms | 1/s | m | RU | RU2 | |
| SSURE1–5_HEPES | 1.87E+4 | 3.63E−4 | 1.94E−8 | 175.7 | 4.22 |
| SSURE1–5_HEPES-Ca2+/Mg2+ | 8.35E+4 | 3.40E−4 | 4.07E−9 | 92.96 | 1.68 |
| SSURE1–5_PBS | 1.12E+5 | 6.20E−4 | 5.55E−9 | 89.56 | 8.69 |
| SSURE2 + 3_PBS | 8211 | 1.46E−3 | 1.78E−7 | 209.0 | 22.1 |
| SSURE2_PBS | 9.75E+4 | 2.72E−3 | 2.79E−8 | 152.8 | 8.80 |
| SH13_HEPES | 1.94E+6 | 5.43E−4 | 2.79E−10 | 86.80 | 4.22 |
| SH13_HEPES-Ca2+/Mg2+ | 2.04E+5 | 5.91E−3 | 2.90E−8 | 4207 | 1.68 |
| SH13_PBS | 7.88E+5 | 8.66E−4 | 1.10E−9 | 132.8 | 1.55 |
| SH2_PBS | 2.28E+4 | 2.15E−3 | 9.43E−8 | 103.0 | 91.6 |
| SH3_PBS | 1.06E+4 | 2.43E−3 | 2.29E−7 | 27.13 | 3.52 |
| SM1_PBS | 7914 | 1.70E−3 | 2.14E−7 | 47.79 | 12.8 |