FIGURE 3.

Z-551 improves insulin sensitivity in the liver and skeletal muscle of WT mice. 4-week-old male WT mice were acclimatized to the HFD for 1 week followed by administration of Z-551 for 15 weeks. A and B, insulin-stimulated Akt (Ser-473) phosphorylation in the liver (A) and skeletal muscle (B) of WT mice on the HFD (white) or HFD+Z-551 (black). The phosphorylation and amount of Akt were analyzed by Western blot (upper panel) and the ratio of phosphorylated Akt to Akt (pAkt/Akt) is calculated (lower panel). All results are expressed as mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.01.