Z-551 indicates therapeutic effects on diet-induced obesity and metabolic disorders in WT DIO mice-Experiment 2. 4-week-old WT mice were given the HFD for 10 weeks and then administered Z-551 for 8 weeks. A and B, changes in body weight (A), and epididymal WAT weight (B) in WT mice on the ND (gray), and WT DIO mice on the HFD (white) or HFD+Z-551 (black). C and D, morphology of epididymal WAT (upper panel) and histogram of adipocyte size from epididymal WAT (lower panel) (C), and average size of adipocytes from epididymal WAT (D). Scale bars indicate 200 μm. E, mRNA expression levels in epididymal WAT. All results are expressed as mean ± S.E. (n = 8–10). *, p < 0.05; **, p < 0.01 compared with the obese mice on the HFD; n.d., not detected.