FIGURE 10.

Lipid body formation in KO-MEFs induced by l-Ser deficiency. KO-MEFs were cultured under l-Ser-supplemented or -deprived conditions for 24 h (B) or 48 h (A and C). A, lipid bodies were stained with Nile Red and observed under a confocal microscope. B, after incubation, KO-MEFs were fixed and observed under an electron microscope. In l-Ser-deprived group, lipid bodies having lamellar inclusions increased (arrows). Asterisk indicates autophagosomes. C, following incubation of KO-MEFs for 48 h, lipids extracted from lipid bodies were analyzed using LC-MS. The data are means ± S.E. (+l-Ser, n = 3; −l-Ser, n = 4). The differences between the two groups were analyzed using the Student's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).