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. 2015 Apr 22;290(23):14595–14609. doi: 10.1074/jbc.M114.603860

FIGURE 9.

FIGURE 9.

Effect of doxSA on KO-MEFs, WT-MEFs, and HeLa cells. KO-MEFs (A and B), WT-MEFs (C), or HeLa (D) cells were cultured for up to 48 h in a medium containing 400 μm l-Ser with either SA (5 μm) or doxSA (0.01, 0.1, 1, or 5 μm). A, viable cell numbers of KO-MEFs treated with 5 μm SA (solid line with closed squares), 5 μm doxSA (dotted line with closed squares), and the vehicle control (open squares) in the presence of l-Ser. B, viable cell numbers of KO-MEFs treated with 0.01 μm (closed squares), 0.1 μm (closed triangles), and 1 μm (closed circles) of doxSA in the presence of l-Ser. The open symbols show viable cell numbers of KO-MEFs treated with the corresponding concentration of vehicle control in the presence of l-Ser. C and D, proliferation of WT-MEFs (C) or HeLa (D) cells treated with 1 μm (closed circles) and 5 μm doxSA (closed diamonds). The open symbols show viable cell numbers of cells treated with the corresponding concentration of vehicle control in the presence of l-Ser. Cell viability was assessed using the trypan blue exclusion method. The data are means ± S.E. from three independent experiments. Differences were analyzed using one-way analysis of variance followed by the Tukey-Kramer test (††, p < 0.01). Differences between the two groups were analyzed using the Student's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).