TABLE 3.
Amino acid concentrations and doxSA contents in the MEFs
| MEFs | Medium | Culture time | Alaa | Sera | Ala/Ser | doxSA productionb |
|---|---|---|---|---|---|---|
| h | ||||||
| WT | +l-Ser | 6 | 2.72 | 6.39 | 0.4 | − |
| −l-Ser | 2.33 | 4.44 | 0.5 | − | ||
| KO | +l-Ser | 3.24 | 2.84 | 1.1 | 1.0 | |
| −l-Ser | 3.25 | 0.98 | 3.3 | 10.3 | ||
| WT | +l-Ser | 24 | 5.64 | 4.45 | 1.3 | 0.7 |
| −l-Ser | 5.51 | 3.46 | 1.6 | 2.5 | ||
| KO | +l-Ser | 3.88 | 1.84 | 2.1 | 0.6 | |
| −l-Ser | 3.00 | 0.82 | 3.7 | 18.0 |
a Intracellular amino acid concentrations (nmol/109 cells) are shown as means (n = 3).
b The amounts of doxSA in WT or KO-MEFs in each condition shown in Fig. 3 were normalized to that obtained from KO-MEFs maintained under the l-Ser-supplemented condition for 6 h. −, not determined.