TABLE 3.
Steady-state kinetic analysis of the effect of SSB on RNase HI activity
| RNase HI | SSB | Km | kcat | kcat/Km | -Fold stimulation |
|---|---|---|---|---|---|
| μm | s−1 | s−1 μm−1 | |||
| Wild type | None | 5.99 ± 0.90 | 1.86 ± 0.14 | 0.31 ± 0.05 | |
| Wild type | Wild type | 0.56 ± 0.11 | 0.69 ± 0.03 | 1.22 ± 0.23 | 3.91 ± 1.00 |
| Wild type | SSBΔF | 3.72 ± 0.66 | 1.63 ± 0.12 | 0.44 ± 0.08 | 1.41 ± 0.10 |
| K3A | None | 4.04 ± 0.52 | 0.64 ± 0.03 | 0.16 ± 0.02 | |
| K3A | Wild type | 2.03 ± 0.13 | 0.79 ± 0.02 | 0.39 ± 0.03 | 2.47 ± 0.38 |
| K3E | None | 5.42 ± 0.57 | 1.50 ± 0.07 | 0.28 ± 0.03 | |
| K3E | Wild type | 3.46 ± 0.54 | 2.03 ± 0.11 | 0.59 ± 0.10 | 2.11 ± 0.43 |
| K60A | None | 5.67 ± 0.89 | 1.76 ± 0.12 | 0.31 ± 0.05 | |
| K60A | Wild type | 0.94 ± 0.10 | 1.47 ± 0.05 | 1.56 ± 0.17 | 5.05 ± 1.03 |
| K60E | None | 2.39 ± 0.48 | 1.08 ± 0.08 | 0.45 ± 0.10 | |
| K60E | Wild type | 1.20 ± 0.21 | 0.98 ± 0.04 | 0.81 ± 0.15 | 1.79 ± 0.50 |