Inhibition of adipocyte differentiation by CD45+CD11b+CD11c+ myeloid cells.
A, increased adipogenesis in preadipocytes treated with BM-DC-conditioned medium from Mmp12−/− mice. B, increased adipogenesis in preadipocytes treated with conditioned medium from BM-DCs in which Fn1 had been silenced. Both experiments were repeated four times, and representative experiments are presented. C, inhibition of adipogenesis when preadipocytes are co-cultured with CD45+CD11b+CD11c+ cells. The experiment was repeated three times. D–F, panel of adipogenesis genes for the CD45+CD11b+CD11c+ co-culture experiments.